Yeast - Reference Chromosome VII

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Nature 387 (suppl.) 81-84 (1997)

 

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII

 

H. Tettelin1, M. L. Agostoni Carbone2, K. Albermann3, M. Albers4, J. Arroyo5, U. Backes4, T. Barreiros6, I. Bertani7, A. J. Bjourson8, M. Brückner9, C.V. Bruschi7, G. Carignani10, L. Castagnoli11, E. Cerdan12, M. L. Clemente10, A. Coblenz4, M. Coglievina7, E. Coissac13, E. Defoor14, S. Del Bino1, H. Delius15, D. Delneri7, P. de Wergifosse1, B. Dujon16, P. Durand17, K. D. Entian18, P. Eraso19, V. Escribano19, L. Fabiani20, B. Fartmann21*, F. Feroli10, M. Feuermann22, L. Frontali20, M. García-Gonzalez5*, M. I. García-Sáez5, A. Goffeau1, P. Guerreiro6, J. Hani3, M. Hansen4, U. Hebling15, K. Hernandez23, K. Heumann3, F. Hilger17, B. Hofmann15, K. J. Indge24, C.M. James24, R. Klima7, P. Kötter18, B. Kramer21*, W. Kramer21, G. Lauquin25, H. Leuther4, E. J. Louis26, E. Maillier13, A. Marconi20, E. Martegani27, M. J. Mazón19, C. Mazzoni20, A. D. K. McReynolds8, P. Melchioretto2, H. W. Mewes3, O. Minenkova11, S. Müller-Auer9, A. Nawrocki28, P. Netter13, R. Neu4, C. Nombela5, S.G. Oliver24, L. Panzeri2, S. Paoluzi11, P. Plevani2, D. Portetelle17, F. Portillo19, S. Potier22, B. Purnelle1, M. Rieger9, L. Riles29, T. Rinaldi20, J. Robben14, C. Rodrigues-Pousada6, E. Rodriguez-Belmonte12, A. M. Rodriguez-Torres12, M. Rose18, M. Ruzzi30, M. Saliola20, M. Sánchez-Perez5, B. Schäfer4, M. Schäfer9, M. Scharfe31, T. Schmidheini23, A. Schreer4, J. Skala28, J. L. Souciet22, H.Y. Steensma32,33, E. Talla1, A. Thierry16, M. Vandenbol17, Q. J. M. van der Aart32, L. Van Dyck1, M. Vanoni27, P. Verhasselt14, M. Voet14, G. Volckaert14, R. Wambutt31, M. D. Watson34, N. Weber23, E. Wedler31, H. Wedler31, P. Wipfli23, K. Wolf4, L. F. Wright8, P. Zaccaria7, M. Zimmermann4, A. Zollner3 & K. Kleine3

 

1 Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix-du-Sud 2/20, B-1348 Louvain-la-Neuve, Belgium

2 Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Via Celoria 26, I-20133 Milano, Italy

3 Martinsrieder Institut für Protein Sequenzen, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany

4 Institut für Biologie IV, Mikrobiologie, Worringerweg, RWTH-Aachen, D-52056, Germany

5 Departamento de Microbiología II and Centro de Secuenciación de DNA de la UCM, Faculdad de Farmacia, Universidad Complutense, E-28040 Madrid, Spain

6 Laboratorio de Genetica Molecular, Instituto Gulbenkian de Ciencia, Ap 14, E-2781 Oeiras Codex, Portugal

7 Microbiology Group, International Center for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy

8 The Biotechnology Center for Animal and Plant Health, The Queens University of Belfast, Newforge Lane, Belfast, BT9 5PX, UK

9 Genotype GmbH, Angelhofweg 39, D-69259 Wilhelmsfeld, Germany

10 Dipartimento di Chimica biologica, Università di Padova, via Trieste 75, I-35121 Padova, Italy

11 Dipartimento di Biologia, Università di Roma ‘Tor Vergata’, via della Ricerca Scientifica, I-00133 Roma, Italy

12 Departamento de Biologia Celular y Molecular, Facultad de Ciencias, Universidad de La Coruna, Campus de La Zapateira s/n, La Coruna, Spain

13 Centre de Génétique Moléculaire, CNRS, Laboratoire Associé à l’Université Pierre et Marie Curie, F-91198 Gif-sur-Yvette Cedex, France

14 Katholieke Universiteit Leuven, Laboratory of Gene Technology, Willem de Croylaan 42, B-3001 Leuven, Belgium

15 Deutsches Krebsforschungszentrum, Department for Applied Virology, D-69120 Heidelberg, Germany

16 Unité de Génétique Moléculaire des levures (URA 1149 of CNRS and UPR 927 of University P.M. Curie, Paris), Department of Biotechnologies, 25 rue du Dr. Roux, Institut Pasteur, F-75724 Paris Cedex 15, France

17 Unité de Microbiologie, Faculté Universitaire des Sciences Agronomiques de Gembloux, Avenue Maréchal Juin 6, B-5030 Gembloux, Belgium

18 Institut für Mikrobiologie der Johann Wolfgang Goethe-Universität Frankfurt /M, Marie-Curie Strasse 9, D-60439 Frankfurt, Germany;

19 Instituto de Investigaciones Biomédicas del C.S.I.C.y Departamento de Bioquímica, Facultad de Medicina de la U.A.M., E-28029 Madrid, Spain

20 Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, P. le Aldo Moro 5, I-00185 Roma, Italy

21 Institut für Molekulare Genetik, Georg-August-Universität, Grisebachstr. 8, D-37077 Göttingen, Germany

22 Laboratoire de Microbiologie et Génétique URA 1481, Université Louis Pasteur/CNRS, Institut de Botanique, rue Goethe 28, F-67083 Strasbourg Cedex, France

23 Microsynth GmbH, Schützenstrasse 15, CH-9436 Balgach, Switzerland

24 Department of Biochemistry and Applied Molecular Biology, UMIST, PO Box 88, Sackville St., Manchester M60 1QD, UK

25 Institut de Biochimie Cellulaire, CNRS, rue Camille Saint-Saèns 1, F-33077 Bordeaux Cedex, France

26 Yeast Genetics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DU, UK

27 Dipartimento di Biochimica e Fisiologia Generali, Università di Milano, Via Celoria 26, I-20133 Milano, Italy

28 Institute of Microbiology, Wroclaw University, Przybyszewskiego 63, P-51148 Wroclaw, Poland

29 Genome Sequencing Center and Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA

30 Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, via S. Camillo de Lellis, I-01100 Viterbo, Italy

31 AGON GmbH, Glienicker Weg 185, D-12489 Berlin, Germany

32 Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, NL-2333 AL Leiden, The Netherlands

33 Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands

34 Department of Biological Sciences, University of Durham, South Road, Durham, DH1 3LE, UK

 

*Present addresses: MWG-BIOTECH GmbH, Anzinger Strasse 7, D-85560 Ebersberg, Germany (B. F.); Centro de Biotecnología, Camagüey, Cuba (M. G.); Abteilung Klinische Biochemie, Zentrum Innere Medizin, Georg-August-Universität, Robert-Koch-Strasse 40, D-37075 Göttingen, Germany, (B. K.).

 

The complete nucleotide sequence of Saccharomyces cerevisiae chromosome VII has 572 predicted open reading frames (ORFs), of which 341 are new. No correlation was found between GC content and gene density along the chromosome, and their variations are random. Of the ORFs, 17% show high similarity to human proteins. Almost half of the ORFs could be classified in functional categories and there is a slight increase in the number of transcription (7.0 %) and translation (5.2 %) factors when compared with the complete S. cerevisiae genome. Accurate verification procedures demonstrate that there are less than 2 errors per 10,000 base pairs in the published sequence.

 


EMBL accession numbers: X94357, Z72785-Z73081