|Yeast - Reference Chromosome IX|
C. Churcher1, S. Bowman1, K. Badcock1, A. Bankier2, D. Brown1, T. Chillingworth1, R. Connor1, K. Devlin1, S. Gentles1, N. Hamlyn1, D. Harris1, T. Horsnell2, S. Hunt1, K. Jagels1, M. Jones1, G. Lye1, S. Moule1, C. Odell1, D. Pearson1, M. Rajandream1, P. Rice1, N. Rowley2, J. Skelton1, V. Smith2, S. Walsh1, S. Whitehead1 & B. Barrell1.
1 The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
2 MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, several groups have successfully demonstrated the viability of the whole-genome random ‘shotgun’ method in large-scale sequencing of both viruses and prokaryotes1–5. Here, we report the sequence of Saccharomyces cerevisiae chromosome IX, determined in part by a whole-chromosome ‘shotgun’, and describe the particular difficulties encountered in the random ‘shotgun’ sequencing of an entire eukaryotic chromosome. Analysis of this sequence shows that chromosome IX contains 221 open reading frames (ORFs), of which approximately 30% have been sequenced previously. This chromosome shows features typical of a small Saccharomyces cerevisiae chromosome.