|Yeast - Reference Chromosome XIII|
S. Bowman, C. Churcher, K. Badcock, D. Brown, T. Chillingworth, R. Connor, K. Devlin, S. Gentles, N. Hamlyn, S. Hunt, K. Jagels, G. Lye, S. Moule, C. Odell, D. Pearson, M. Rajandream, P. Rice, J. Skelton, S. Walsh, S. Whitehead & B. Barrell.
The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 15A, UK.
Systematic sequencing of the genome of Saccharomyces cerevisiae has revealed thousands of new predicted genes and allowed analysis of long-range features of chromosomal organization. Generally, genes and predicted genes seem to be distributed evenly throughout the genome, having no overall preference for DNA strand. Apart from the smaller chromosomes, which can have substantially lower gene density in their telomeric regions1-3, there is a consistent average of one open reading frame (ORF) approximately every 2 kilo bases. However, one of the most surprising findings for a eukaryote with approximately 6,000 genes was the amount of apparent redundancy in its genome. This redundancy occurs both between individual ORFs, and over more extensive chromosome regions, which have been duplicated preserving gene order and orientation4-6. Here we report the entire nucleotide sequence of chromosome XIII, the sixth-largest S. cerevisiae chromosome, and demonstrate that its features and organization are consistent with those observed for other S. cerevisiae chromosomes. Analysis revealed 459 ORFs; of which 284 have not been identified previously. Both intra- and interchromosomal duplications of regions of this chromosome have occurred.