Yeast - Reference Chromosome XIV

mips


Nature 387 (suppl.) 93-98 (1997)

 

The complete nucleotide sequence of Saccharomyces cerevisiae chromosome XIV

 

P. Philippsen1,2, K. Kleine3, R. Pöhlmann1, A. Düsterhöft4, K. Hamberg2, J. H. Hegemann2, B. Obermaier5,6, L. A. Urrestarazu7, R. Aert8, K. Albermann3, R. Altmann1, B. André7, V. Baladron9, J. P. G. Ballesta10, A.-M. Bécam11, J. Beinhauer2, J. Boskovic10, M. J. Buitrago9, F. Bussereau12, F. Coster13, M. Crouzet14, M. D’Angelo15, F. Dal Pero15, A. De Antoni15, F. Del Rey9, F. Doignon14, H. Domdey5, E. Dubois16, T. Fiedler2, U. Fleig2, M. Floeth4, C. Fritz4, C. Gaillardin17, J. M. Garcia-Cantalejo10, N. N Glansdorff16, A. Goffeau13, U. Gueldener2, C. Herbert11, K. Heumann3, D. Heuss-Neitzel4, H. Hilbert4, K. Hinni1, I. Iraqui Houssaini7, M. Jacquet12, A. Jimenez10, J.-L. Jonniaux13, L. Karpfinger3, G. Lanfranchi15, A. Lepingle17, H. Levesque17, R. Lyck2, M. Maftahi17, L. Mallet12, K. C. T. Maurer18, F. Messenguy16, H. W. Mewes3, D. Möstl4, F. Nasr11, J.-M. Nicaud17, R. K. Niedenthal2, D. Pandolfo15, A. Piérard16, E. Piravandi5, R. J. Planta18, T. M. Pohl19, B. Purnelle13, C. Rebischung1, M. Remacha10, J. L. Revuelta9, M. Rinke5, J. E. Saiz9, F. Sartorello15, B. Scherens16, M. Sen-Gupta2, A. Soler-Mira10, J. H. M. Urbanus18, G. Valle15, L. Van Dyck13, P. Verhasselt8, F. Vierendeels16, S. Vissers7, M. Voet8, G. Volckaert8, A. Wach1, R. Wambutt20, H. Wedler20, A. Zollner3 & J. Hani3.

 

1 Institute for Applied Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

2 Justus-Liebig-Universität Giessen, Institut für Mikro-und Molekularbiologie, Frankfurter Strasse 107, D-35392 Giessen, Germany

3 Martinsried Institut für Protein Sequenzen, am Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany

4 QIAGEN GmbH, Max-Volmer-Strasse 4, D-40724 Hilden, Germany

5 Ludwig-Maximilians Universität, Laboratorium für Molekulare Biologie, Genzentrum der LMU München, Würmtalstrasse 221, D-81375 München, Germany

6 MediGene GmbH, Lochhamer Strasse 11, D-82152 Martinsried, Germany

7 Université Libre de Bruxelles, Physiologie Cellulaire et Génétique des Levures, Boulevard du Triompe CP244, B-1050 Bruxelles, Belgium

8 Katholieke Universiteit Leuven, Laboratory of Gene Technology, Willem de Croylaan 42, B-3001 Leuven, Belgium

9 Departamento de Microbiologia y Genética, Universidad de Salamanca, Avenida del Campo Charro s/n, E-37007 Salamanca, Spain

10 Centro de Biologia Molecular, CSIC & UAM, Cantoblanco, E-28049 Madrid, Spain

11 Centre de Génétique Moléculaire, Laboratoire propre du CNRS associé à l’Université Pierre et Marie Curie, F-91198 Gif-sur Yvette, France

12 Université Paris-Sud, Institut de Génétique et Microbiologie, Laboratoire Information Génétique et Développement, Bât. 400, F-91405 Orsay Cedex, France

13 Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2/20, B-1348 Louvain-la-Neuve, Belgium

14 LBMS, Université de Bordeaux 2, UPR CNRS 9026, BP 64, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France

15 Department of Biology, CRIBI Biotechnology Centre, University of Padova, via Trieste, 75, I-35121 Padova, Italy

16 CERIA-COOVI, Avenne E. Gryson 1, B-1070 Brussels, Belgium

17 Institut National Agronomique Paris-Grignon, Laboratoire de Génétique Moléculaire et Cellulaire, Centre de Biotechnologies Agro-Industrielles, F-78850 Thiverval-Grignon, France

18 Department of Biochemistry and Molecular Biology, IMBW, BioCentrum Amsterdam, Vrije Universiteit de Boelelaan 1083, NL-1081 HV Amsterdam, Netherlands

19 AGON GmbH, Glienicker Weg 185, D-12498 Berlin, Germany

20 GATC-Gesellschaft für Analyse-Technik und Consulting mbH, Fritz-Arnold-Strasse 23, D-78467 Konstanz, Germany

 

In 1992 we started assembling an ordered library of cosmid clones from chromosome XIV of the yeast Saccharomyces cerevisiae. At that time, only 49 genes were known to be located on this chromosome1 and we estimated that 80% to 90% of its genes were yet to be discovered. In 1993, a team of 20 European laboratories began the systematic sequence analysis of chromosome XIV. The completed and intensively checked final sequence of 784,328 base pairs was released in April, 1996 (ref. 2). Substantial parts had been published before3-22 or had previously been made available on request. The sequence contained 419 known or presumptive protein-coding genes, including two pseudo-genes and three retrotransposons, 14 tRNA genes, and three small nuclear RNA genes. For 116 (30%) protein-coding sequences, one or more structural homologues were identified elsewhere in the yeast genome. Half of them belong to duplicated groups of 6-14 loosely linked genes, in most cases with conserved gene order and orientation (relaxed interchromosomal synteny). We have considered the possible evolutionary origins of this unexpected feature of yeast genome organization.



EMBL accession numbers: Z71277-Z71692