Yeast - Reference Chromosome XV

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Nature 387 (suppl.) 98-102 (1997)

 

The complete nucleotide sequence of yeast chromosome XV

 

B. Dujon1, K. Albermann2, M. Aldea3, D. Alexandraki4,5, W. Ansorge6, J. Arino7, V. Benes6, C. Bohn8, M. Bolotin-Fukuhara8, R. Bordonné9, J. Boyer1, A. Camasses9, A. Casamayor7, C. Casas3, G. Chéret10, C. Cziepluch11, B. Daignan-Fornier8, D. V. Dang8, M. de Haan12, H. Delius13, P. Durand14, C. Fairhead1, H. Feldmann15, L. Gaillon1, F. Galisson1, F.-J. Gamo16, C. Gancedo16, A. Goffeau17, S. E. Goulding18, L. A. Grivell12, B. Habbig19, N. J. Hand18, J. Hani2, U. Hattenhorst19, U. Hebling13, Y. Hernando20, E. Herrero3, K. Heumann2, R. Hiesel21, F. Hilger14, B. Hofmann13, C. P. Hollenberg19, B. Hughes22, J.-C. Jauniaux11, A. Kalogeropoulos8, C. Katsoulou4, E. Kordes11, M. J. Lafuente16, O. Landt23, E. J. Louis24 C. Maarse12, A. Madania9, G. Mannhaupt15, C. Marck25, R. P. Martin9, H. W. Mewes2, G. Michaux1, V. Paces26, A. G. Parle-McDermott18, B. M. Pearson20, A. Perrin1, B. Pettersson27, O. Poch9, T. M. Pohl22, R. Poirey11, D. Portetelle14, A. Pujol11, B. Purnelle17 M. Ramezani Rad19, S. Rechmann6, C. Schwager6, M. Schweizer20, F. Sor10, F. Sterky27, I. A. Tarassov9, C. Teodoru6, H. Tettelin1,17, A. Thierry1, E. Tobiasch11, M. Tzermia4, M. Uhlen27, M. Unseld21, M. Valens8, M. Vandenbol14, I. Vetter15, C. Vleck26, M. Voet28, G. Volckaert28, H. Voss6, R. Wambutt29, H. Wedler29, S. Wiemann6, B. Winsor9, K. H. Wolfe18, A. Zollner2, E. Zumstein20 & K. Kleine2.

 

1 Unité de Génétique Moléculaire des Levures (URA 1149 CNRS and UFR 927 Univ. P.M. Curie), Institut Pasteur, 25 Rue du Dr. Roux, F75724, Paris Cedex 15, France

2 Martinsrieder Institut für Protein Sequenzen, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152, Martinsried, Germany

3 Department of Basic Medical Sciences, Faculty of Medicine, University of Lleida, E-25006, Lleida, Spain

4 Foundation for Research and Technology-Hellas, IMBB, P.O. Box 1527, Heraklion 711 10 Crete, Greece

5 Department of Biology, University of Crete, Heraklion 711 10 Crete, Greece

6 Biochemical Instrumentation Program, EMBL, Meyerhofstrasse 1, D-69117, Heidelberg, Germany

7 Departamento de Bioquimica y Biologia Molecular, Universidad Autonoma de Barcelona, Bellaterra, E-08193, Spain

8 Institut de Génétique et Microbiologie, Bâtiment 400, Université Paris-Sud, F-91405 Orsay Cedex, France

9 UPR9005 (MMDCD) du CNRS, 15 rue René Descartes, F-67084, Strasbourg, France

10 Institut Curie, Bâtiment 110, Centre Universitaire, F-91405, Orsay Cedex, France

11 Tumorvirologie Abteilung 0610 and Virologie appliquée à l’oncologie (INSERM U375), Deutsches Krebsforschungszentrum, P.101949, D-69009, Heidelberg, Germany

12 Section for Molecular biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, The Netherlands

13 Abteilung für Angewandte Tumorvirologie 0686, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 506, D-69120 Heidelberg, Germany

14 Faculté Universitaire des Sciences Agronomiques, Unité de Microbiologie, 6 Avenue Maréchal Juin, B-5030 Gembloux, Belgium

15 Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Goethestrasse 33, D-80336 München, Germany

16 Instituto de Investigaciones Biomedicas, CSIC, Arturo Duperier 4, E-28029 Madrid, Spain

17 Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2-20, B-1348 Louvain-La-Neuve, Belgium

18 Department of Genetics, Univeristy of Dublin, Trinity College, Dublin 2, Ireland

19 Institut für Mikrobiologie der Heinrich-Heine-Universität Düsseldorf, Geb. 26.12, Universitätstrasse 1 D-40225 Düsseldorf, Germany

20 Institute of Food Research, Genetics and Microbiology Department, Norwich Research Park, Colney, Norwich NR4 7UA, UK

21 Institut für Genbiologische Forschung, Ihnestrasse 63, D-14195 Berlin, Germany

22 GATC-Gesellschaft für Analyse-Technik und Consulting mbH, Fritz-Arnold-Strasse 23, Konstanz, D-78467, Germany

23 TIB MolBiol, Tempelhofer Weg 11-12, Berlin, D-10829 Germany

24 Yeast Genetics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, UK

25 Service de Biochimie et de Génétique moléculaire, Département de Biologie Cellulaire et Moléculaire, DSV/CEA-Saclay, F-91191 Gif-sur-Yvette, France

26 Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech republic

27 Department of Biochemistry and Biotechnology, Royal Institute of Technology (KTH). S10044, Stockholm, Sweden

28 Laboratory of Gene Technology, Katholieke Universiteit Leuven, Willem de Croylaan, 42, B-3001, Leuven, Belgium

29 AGON GmbH, Glienicker Weg 185, D-12489 Berlin, Germany,

 

Chromosome XV was one of the last two chromosomes of Saccharomyces cerevisiae to be discovered1. It is the third-largest yeast chromosome after chromosomes XII and IV, and is very similar in size to chromosome VII. It alone represents 9 % of the yeast genome (8 % if one includes ribosomal DNA is included). When systematic sequencing of chromosome XV was started, 93 genes or markers were identified, and most of them were mapped2. However, very little else was known about chromosome XV which, in contrast to shorter chromosomes, had not been the object of comprehensive genetic or molecular analysis. It was therefore decided to start sequencing chromosome XV only in the third phase of the European Yeast Genome Sequencing Programme, after experience was gained on chromosomes III, XI and II (refs 3-5). The sequence of chromosome XV has been determined from a set of partly overlapping cosmid clones derived from a unique yeast strain, and physically mapped at 3.3 kilobase resolution before sequencing. As well as numerous new open reading frames (ORFs) and genes encoding tRNA or small RNA molecules, the sequence of 1,091,283 base pairs confirms the high proportion of orphan genes and reveals a number of ancestral and successive duplications with other yeast chromosomes.

 


EMBL accession numbers: Z74743-Z75302