Yeast - Physiological and Genetic Pathways

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DNA non-homologous-end-joining: from yeast to man

Plasmid-repair products in non-homologous-end-joining (NHEJ)-mutant yeast strains. Schematic representation of the relative accuracy and efficiency of plasmid rejoining in Saccharomyces cerevisiae strains in which various components of the NHEJ apparatus are disrupted. DSB, double-strand break.

For detailed information click the appropriate protein or complex in the figure or table and see:

Susan E. Critchlow and Stephen P. Jackson
DNA end-joining: from yeast to man
Trends Biochem. Sci. 23: 394-398 (1998)


Figure and table were kindly provided by Stephen P. Jackson




Human

Yeast

Properties

Ku70

Ku80 (Ku86)

Yku70p (Hdf1p)

Yku80p (Hdf2p)

The Ku70/Ku80 heterodimer comprises the DNA end-binding component of DNA-PK; yeast and mammalian homologues required for efficient NHEJ

DNA-PKcs

 

Confers DNA-dependent protein kinase catalytic function (~465 kDa); related to ATM, ATR, Mec1p and Tel1p; no clear yeast homologue

XRCC4

Lif1p

Strong and specific interaction with DNA ligase IV; human XRCC4 stimulates DNA ligase IV activity in vitro; Lif1p stabilises Lig4p

DNA ligaseIV

Lig4p (Dnl4p)

DNA end-joining activity; human and yeast homologues associate with XRCC4 or Lif1p, respectively; yeast homologue functions in Ku-dependent NHEJ pathway

RAD50

Rad50p

Yeast and human homologues interact with Mre11; shares homology with the Escherichia coli ATP-dependent exonuclease SbcC

MRE11

Mre11p

Shares homology with the E. coli ATP-dependent exonuclease SbcD; Mre11 exhibits 3' to 5' exonuclease and hairpin endonuclease activity

NBS1

 


Xrs2p

NBS1 interacts with the Mre11/Rad50 complex and is likely to be the functional homologue of Xrs2p

Xrs2p interacts with Mre11p in a yeast two-hybrid system

SIR2

Sir2p

(Hst1-4p)

Sir3p, Sir4p

Identified through its function in transcriptional silencing; forms a complex with Sir3p and Sir4p


Sir4p interacts with Yku70p in yeast two-hybrid system

Abbreviations used: DNA-PKcs, catalytic subunit of the DNA-dependent protein kinase; Lif, ligase-interacting factor; NBS, Nijmegan breakage syndrome; Sir, silencing information regulator.

Generated Mon Feb 1 1999 Last modified: Tue Feb 10 10:14:46 CET 2004