Yeast - Physiological and Genetic Pathways


Early steps of protein translocation into the endoplasmatic reticulum

Click the appropriate protein or complex name to get information about the corresponding protein or complex.
This page was kindly provided by Randy Schekman
Pilon M, Roemisch K, Quach D and Schekman R
Sec61p serves multiple roles in secretory precursor binding and translocation into the endoplasmatic reticulum membrane.
Molecular Biology of the Cell 9: 3455-3473 (1998)

Cs sec61 mutants define two early stages of posttranslational translocation. The trimeric Sec61p complex consists of Sec61p and two smaller proteins, Sbh1p and Ssp1p. The Sec61p complex and Sec62/Sec63p complex assemble to form the heptameric Sec complex (1), which binds precursor at a cytoplasmic docking site (2). After Kar2p recruitment and ATP hydrolysis (3), precursor is released from the docking site and inserts into the pore (4). Kar2p directly promotes the translocation (5) and release of precursors into the lumen (6). The drawing does not represent all the protein-protein interactions to occur.
Generated Thu January 21 1999 Last modified: Tue Feb 10 10:25:15 CET 2004